Saturday, 5 November 2016

Important stains and their uses in pathology.

Important stains and their uses in pathology.
1. Lipid or fat - Sudan black or oil red O
2. Calcium - von kossa or alzarine red s
3. Haemosiderin or iron - Prussian blue or Perls stain
4. Collagen - Masson trichrome
5. Mucin - mucicarmine, alcian blue
6. Fungi - PAS, silver methanamine
7. Melanin - Masson Fontana
8. Mast cells - toluidine blue
9. Amyloid - Congo red
10. Basement membrane - PAS
11. Nucleic acid or DNA - feulgen, acridine orange
12. Routine histo pathology - hematoxylin and eosin ( H&E)
13. Routine peripheral smear - geimsa or leishman
14. Lymphoblast - PAS ( block positivity)
15. Myeloblasts - myeloperoxidase, non specific esterase, Sudan black B.

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